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KMID : 0624620090420060338
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BMB Reports
2009 Volume.42 No. 6 p.338 ~ p.343
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β-catenin protein utilized by Tumour necrosis factor-α in porcine preadipocytes to suppress differentiation
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Luo Xiao
Li Hui-Xia
Liu Rong-Xin
Wu Zong-Song
Yang Ying-Juan
Yang Gong-She
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Abstract
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The Wnt/β-catenin signaling pathway alters adipocyte differentiation by inhibiting adipogenic gene expression. β-catenin plays a central role in the Wnt/β-catenin signaling pathway. In this study, we revealed that tumour necrosis factor-α (TNF-α), a potential negative regulator of adipocyte differentiation, inhibits porcine adipogenesis through activation of the Wnt/β-catenin signaling pathway. Under the optimal concentration of TNF-α, the intracellular β-catenin protein was stabilized. Thus, the intracellular lipid accumulation of porcine preadipocyte was suppressed and the expression of important adipocyte marker genes, including peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer binding protein-α (C/EBPα), were inhibited. However, a loss of β-catenin in porcine preadipocytes enhanced the adipogenic differentiation and attenuated TNF-α induced anti-adipogenesis. Taken together, this study indicated that TNF-α inhibits adipogenesis through stabilization of β-catenin protein in porcine preadipocytes.
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KEYWORD
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β-catenin, Differentiation, Porcine preadipocytes, TNF-α, Wnt/β-catenin signaling pathway
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